6UFE

The structure of a potassium selective ion channel at atomic resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2017-06-06
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97
Spacegroup name	I 4
Unit cell lengths	67.860, 67.860, 89.620
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	21.459 - 1.200
R-factor	0.1331
Rwork	0.133
R-free	0.14770
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ouf
RMSD bond length	0.030
RMSD bond angle	4.090
Data reduction software	XDS
Data scaling software	XSCALE
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.230
High resolution limit [Å]	1.200	1.200
Number of reflections	61537	3673
<I/σ(I)>	12.23
Completeness [%]	97.4
Redundancy	5.34
CC(1/2)	0.998	0.489

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	See paper for details