6UF6

Crystal structure of B. subtilis TagU

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-10-05
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97949
Spacegroup name	P 32 2 1
Unit cell lengths	48.792, 48.792, 234.271
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.255 - 2.200
R-factor	0.2452
Rwork	0.244
R-free	0.25880
Structure solution method	SAD
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.255	2.279
High resolution limit [Å]	2.200	2.200
Number of reflections	17406	1686
<I/σ(I)>	16.23	1.55
Completeness [%]	99.6	99.11
Redundancy	2	2
CC(1/2)	0.998	0.897

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	1.6 M ammonium sulfate