6UF5

Crystal structure of B. subtilis TagT

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-02-22
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97964
Spacegroup name	P 41 21 2
Unit cell lengths	65.656, 65.656, 143.070
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.371 - 2.800
R-factor	0.2333
Rwork	0.231
R-free	0.27950
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4de9
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.371	2.900
High resolution limit [Å]	2.800	2.800
Number of reflections	8240	795
<I/σ(I)>	13.1	1.55
Completeness [%]	99.8
Redundancy	13.9
CC(1/2)	0.997	0.655

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	298	0.2 M sodium malonate, 0.1 M Bis-tris propane pH 6.5, 20% PEG 3350