6UF5
Crystal structure of B. subtilis TagT
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-02-22 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.97964 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 65.656, 65.656, 143.070 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.371 - 2.800 |
R-factor | 0.2333 |
Rwork | 0.231 |
R-free | 0.27950 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4de9 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.371 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Number of reflections | 8240 | 795 |
<I/σ(I)> | 13.1 | 1.55 |
Completeness [%] | 99.8 | |
Redundancy | 13.9 | |
CC(1/2) | 0.997 | 0.655 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | 0.2 M sodium malonate, 0.1 M Bis-tris propane pH 6.5, 20% PEG 3350 |