6UF1

Pistol ribozyme transition-state analog vanadate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-02-21
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	1.89170
Spacegroup name	P 42 21 2
Unit cell lengths	90.570, 90.570, 122.558
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.981 - 3.100
R-factor	0.2048
Rwork	0.199
R-free	0.25470
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	122.600	3.080
High resolution limit [Å]	2.900	2.900
Rpim	0.046	5.326
Number of reflections	10544
<I/σ(I)>	11.2
Completeness [%]	98.9
Redundancy	8.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	0.05 M HEPES sodium, pH 7.5, 0.1 M manganese chloride, 25% MPD