6UEX
Crystal structure of S. aureus LcpA in complex with octaprenyl-pyrophosphate-GlcNAc
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-01-10 |
Detector | RAYONIX MX-300 |
Wavelength(s) | 0.97949 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 89.245, 90.543, 94.753 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.377 - 1.900 |
R-factor | 0.1939 |
Rwork | 0.192 |
R-free | 0.22580 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3nxh |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.380 | 1.968 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.050 | 1.514 |
Number of reflections | 30587 | 3021 |
<I/σ(I)> | 16.31 | 1.36 |
Completeness [%] | 99.8 | |
Redundancy | 6.6 | |
CC(1/2) | 0.998 | 0.696 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.2 | 298 | 2.4 M ammonium sulfate, 0.08 M citric acid pH 5.2 |