6UEW

Rubisco / CsoS2 N-peptide complex responsible for alpha-carboxysome cargo loading

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-12-05
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.11
Spacegroup name	C 1 2 1
Unit cell lengths	171.831, 153.945, 108.063
Unit cell angles	90.00, 124.70, 90.00

Refinement procedure

Resolution	104.080 - 2.400
R-factor	0.189
Rwork	0.188
R-free	0.25890
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1svd
RMSD bond length	0.008
RMSD bond angle	0.881
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX (1.13_2998)
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	104.100	2.486
High resolution limit [Å]	2.400	2.400
Rmerge	0.124	0.588
Rmeas	0.135	0.640
Rpim	0.051	0.250
Number of reflections	90035	8960
<I/σ(I)>	12.84	2.97
Completeness [%]	99.9	99.92
Redundancy	6.9	6.4
CC(1/2)	0.997	0.920

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	PEG 400, magnesium chloride, HEPES