6UEO

Structure of A. thaliana TBP-AC mismatch DNA site

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-08-24
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.0
Spacegroup name	P 1
Unit cell lengths	42.242, 55.512, 146.340
Unit cell angles	89.97, 90.03, 90.14

Refinement procedure

Resolution	55.512 - 2.000
R-factor	0.2147
Rwork	0.214
R-free	0.24980
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1qne
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	73.100	2.050
High resolution limit [Å]	2.000	2.000
Rpim	0.057	0.196
Number of reflections	77170	4480
<I/σ(I)>	7.1	2.9
Completeness [%]	90.7
Redundancy	1.7
CC(1/2)	0.998	0.097

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.1 M Sodium acetate, 38% ethylene glycol