6UEH
Crystal structure of a ruminal GH26 endo-beta-1,4-mannanase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LNLS BEAMLINE W01B-MX2 |
| Synchrotron site | LNLS |
| Beamline | W01B-MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-08-21 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.4586 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 56.769, 53.748, 89.696 |
| Unit cell angles | 90.00, 103.51, 90.00 |
Refinement procedure
| Resolution | 43.607 - 1.849 |
| R-factor | 0.168 |
| Rwork | 0.166 |
| R-free | 0.21580 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3zm8 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.704 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.607 | 1.910 |
| High resolution limit [Å] | 1.849 | 1.849 |
| Number of reflections | 43958 | 4204 |
| <I/σ(I)> | 7.78 | |
| Completeness [%] | 97.2 | |
| Redundancy | 3.5 | |
| CC(1/2) | 0.990 | 0.520 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | PEG 8,000 (18% v/v), PEG 400 (10% v/v), 0.1M sodium acetate pH 5.5, 500 mM sodium chloride, Glycerol (10% v/v) and Dioxane (0.5% v/v) |
