6UEC
Pseudomonas aeruginosa LpxD Complex Structure with Ligand
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2017-11-28 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.9792 |
| Spacegroup name | H 3 |
| Unit cell lengths | 104.764, 104.764, 95.967 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 32.990 - 2.600 |
| R-factor | 0.2128 |
| Rwork | 0.209 |
| R-free | 0.28850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.696 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0253) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.990 | 2.693 |
| High resolution limit [Å] | 2.600 | 2.601 |
| Rmerge | 0.105 | |
| Number of reflections | 12038 | 1223 |
| <I/σ(I)> | 3.4 | |
| Completeness [%] | 99.6 | |
| Redundancy | 2.6 | |
| CC(1/2) | 0.568 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 12% PEG 3350 0.2M Magnesium Acetate |
