6UCR

Structure of ClpC1-NTD L92S L96P

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2019-06-04
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	.97872
Spacegroup name	P 21 21 21
Unit cell lengths	38.137, 63.350, 68.305
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.000 - 2.300
R-factor	0.18899
Rwork	0.186
R-free	0.23697
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6cn8
RMSD bond length	0.014
RMSD bond angle	1.797
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.340
High resolution limit [Å]	2.300	2.300
Rmeas	0.100	0.253
Rpim	0.028	0.068
Number of reflections	7594	376
<I/σ(I)>	56.1	27.2
Completeness [%]	96.4
Redundancy	12.7
CC(1/2)	0.995	0.990

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	0.1 M HEPES, pH 7.5, 10% PEG8000, 8% ethylene glycol