6UCL

Transcription factor deltaFosB bZIP domain self-assembly, type-I crystal

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-10-30
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9787
Spacegroup name	F 2 2 2
Unit cell lengths	39.506, 51.956, 150.454
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.782 - 2.207
R-factor	0.2723
Rwork	0.271
R-free	0.28700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5vpe
RMSD bond length	0.003
RMSD bond angle	0.476
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.230
High resolution limit [Å]	2.190	5.940	2.190
Rmerge	0.059	0.038	0.845
Rmeas	0.066	0.045	0.934
Rpim	0.030	0.023	0.389
Total number of observations	21906
Number of reflections	4096	228	194
<I/σ(I)>	7.3
Completeness [%]	99.6	95	100
Redundancy	5.3	4.7	5.2
CC(1/2)		0.993	0.886

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7	293	17% (w/v) PEG 4000, 0.2 M ammonium sulfate