6UCI

Transcription factor DeltaFosB bZIP domain self-assembly, oxidized form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-11-21
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9787
Spacegroup name	P 21 21 2
Unit cell lengths	72.278, 97.497, 45.197
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.665 - 2.090
R-factor	0.2101
Rwork	0.207
R-free	0.24070
Structure solution method	SAD
RMSD bond length	0.005
RMSD bond angle	0.580
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AutoSol
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.130
High resolution limit [Å]	2.090	5.670	2.090
Rmerge	0.048	0.017	0.746
Rmeas	0.055	0.020	0.865
Rpim	0.027	0.010	0.431
Total number of observations	78088
Number of reflections	19503	1002	958
<I/σ(I)>	14.3
Completeness [%]	99.5	91.7	99.9
Redundancy	4	3.8	3.9
CC(1/2)		0.999	0.670

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	293	2.5 M sodium chloride, 0.1 M sodium acetate, pH 5.0