6UCD

The crystal structure of Staphylococcus aureus super antigen-like protein SSL10

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	110
Detector technology	IMAGE PLATE
Collection date	2010-02-16
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.5418
Spacegroup name	P 43
Unit cell lengths	91.550, 91.550, 46.550
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.780 - 2.850
R-factor	0.248
Rwork	0.246
R-free	0.28100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1v1o
Data reduction software	MOSFLM
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	64.740	3.000
High resolution limit [Å]	2.850	2.850
Rmerge	0.121	0.557
Rmeas	0.147	0.683
Rpim	0.081	0.385
Number of reflections	8204	1139
<I/σ(I)>	6.8	1.6
Completeness [%]	89.5	87.4
Redundancy	2.8	2.5
CC(1/2)	0.980	0.440

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	291	30% PEG1500, 8% MPD, 0.1 M Tris, pH 8.5