6UCD
The crystal structure of Staphylococcus aureus super antigen-like protein SSL10
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 110 |
Detector technology | IMAGE PLATE |
Collection date | 2010-02-16 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.5418 |
Spacegroup name | P 43 |
Unit cell lengths | 91.550, 91.550, 46.550 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.780 - 2.850 |
R-factor | 0.248 |
Rwork | 0.246 |
R-free | 0.28100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1v1o |
Data reduction software | MOSFLM |
Data scaling software | Aimless (0.7.4) |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 64.740 | 3.000 |
High resolution limit [Å] | 2.850 | 2.850 |
Rmerge | 0.121 | 0.557 |
Rmeas | 0.147 | 0.683 |
Rpim | 0.081 | 0.385 |
Number of reflections | 8204 | 1139 |
<I/σ(I)> | 6.8 | 1.6 |
Completeness [%] | 89.5 | 87.4 |
Redundancy | 2.8 | 2.5 |
CC(1/2) | 0.980 | 0.440 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 291 | 30% PEG1500, 8% MPD, 0.1 M Tris, pH 8.5 |