6UCC

Structure of human PACRG-MEIG1 complex (limited proteolysis)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-07-07
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9795
Spacegroup name	P 41 21 2
Unit cell lengths	66.854, 66.854, 158.920
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.270 - 2.600
R-factor	0.2173
Rwork	0.214
R-free	0.28410
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6ndu
RMSD bond length	0.007
RMSD bond angle	0.883
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.270	2.720
High resolution limit [Å]	2.600	2.600
Rmerge	0.283	2.868
Rmeas	0.295	3.131
Rpim	0.082	0.871
Number of reflections	11793	1395
<I/σ(I)>	9.1	1.1
Completeness [%]	100.0	100
Redundancy	12.6	12.7
CC(1/2)	0.996	0.557

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		283	20% glycerol, 0.2M KH2PO4, 20% PEG 8000