6UC5
Fab397 in complex with NPNA peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-11-24 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.03317 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 61.268, 77.492, 87.701 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.660 - 1.750 |
R-factor | 0.193 |
Rwork | 0.190 |
R-free | 0.22500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | homology model |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.780 |
High resolution limit [Å] | 1.750 | 1.750 |
Rpim | 0.031 | 0.339 |
Number of reflections | 41579 | 1315 |
<I/σ(I)> | 24.5 | 1.2 |
Completeness [%] | 95.3 | |
Redundancy | 10.6 | |
CC(1/2) | 0.969 | 0.823 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 0.1 M HEPES, pH 6.62, 22% w/v PEG4000 |