6UBL

Structure of DynF from the Dynemicin Biosynthesis Pathway of Micromonospora chersina

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-06-12
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0332
Spacegroup name	C 2 2 21
Unit cell lengths	59.830, 116.126, 129.877
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.716 - 1.499
R-factor	0.1625
Rwork	0.161
R-free	0.18510
Structure solution method	SIRAS
Data reduction software	XDS (VERSION Jan 26, 2018 BUILT=20180409)
Data scaling software	XSCALE
Phasing software	SHELX (2013/2)
Refinement software	PHENIX (1.16)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	34.716	34.716	1.590
High resolution limit [Å]	1.499	4.460	1.499
Rmerge	0.056	0.029	1.661
Rmeas	0.061	0.031	1.904
Number of reflections	70922	2932	10132
<I/σ(I)>	14.13	52.06	0.63
Completeness [%]	97.6	99.6	87.1
Redundancy	6.204	6.355	3.88
CC(1/2)	0.999	0.999	0.373

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	25% (w/v) PEG 1500, 0.1 M PCB buffer, pH 7.0