6UBD

Crystal structure of a GH128 (subgroup VII) oligosaccharide-binding protein from Trichoderma gamsii (TgGH128_VII)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	LNLS BEAMLINE W01B-MX2
Synchrotron site	LNLS
Beamline	W01B-MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-12-12
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.999960
Spacegroup name	P 21 21 2
Unit cell lengths	67.345, 74.088, 50.811
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.940 - 1.250
R-factor	0.1698
Rwork	0.169
R-free	0.18810
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.015
RMSD bond angle	2.038
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.940	1.330
High resolution limit [Å]	1.250	1.250
Number of reflections	69710	10928
<I/σ(I)>	11.12
Completeness [%]	98.0
Redundancy	6.41
CC(1/2)	0.997	0.822

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	291	MES 0.1 M 20% PEG6000 Lithium chloride 0.2 M