6UBD
Crystal structure of a GH128 (subgroup VII) oligosaccharide-binding protein from Trichoderma gamsii (TgGH128_VII)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | LNLS BEAMLINE W01B-MX2 |
Synchrotron site | LNLS |
Beamline | W01B-MX2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-12-12 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 0.999960 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 67.345, 74.088, 50.811 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.940 - 1.250 |
R-factor | 0.1698 |
Rwork | 0.169 |
R-free | 0.18810 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.015 |
RMSD bond angle | 2.038 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.940 | 1.330 |
High resolution limit [Å] | 1.250 | 1.250 |
Number of reflections | 69710 | 10928 |
<I/σ(I)> | 11.12 | |
Completeness [%] | 98.0 | |
Redundancy | 6.41 | |
CC(1/2) | 0.997 | 0.822 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 291 | MES 0.1 M 20% PEG6000 Lithium chloride 0.2 M |