6UBA

Crystal structure of a GH128 (subgroup VI) exo-beta-1,3-glucanase from Aureobasidium namibiae (AnGH128_VI) in complex with laminaritriose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	LNLS BEAMLINE W01B-MX2
Synchrotron site	LNLS
Beamline	W01B-MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-12
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	1.458570
Spacegroup name	P 21 21 21
Unit cell lengths	121.262, 152.798, 169.423
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.350 - 2.400
R-factor	0.2377
Rwork	0.237
R-free	0.25560
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6ub8
RMSD bond length	0.004
RMSD bond angle	1.276
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0257)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.350	2.540
High resolution limit [Å]	2.400	2.400
Number of reflections	123227	19656
<I/σ(I)>	6.24	1.15
Completeness [%]	99.0
Redundancy	11.9
CC(1/2)	0.990	0.602

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.18 M tri-ammonium citrate, 18% v/v PEG 3350, 0.01 M Cobalt (II) chloride