6UB8

Crystal structure of a GH128 (subgroup VI) exo-beta-1,3-glucanase from Aureobasidium namibiae (AnGH128_VI)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	LNLS BEAMLINE W01B-MX2
Synchrotron site	LNLS
Beamline	W01B-MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-07-26
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	1.458570
Spacegroup name	P 21 21 21
Unit cell lengths	117.993, 151.860, 166.785
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.210 - 1.900
Rwork	0.222
R-free	0.26400
Structure solution method	SIRAS
RMSD bond length	0.013
RMSD bond angle	1.875
Data reduction software	XDS
Data scaling software	XDS
Phasing software	SHELXCD
Refinement software	REFMAC (5.8.0253)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.020
High resolution limit [Å]	1.900	1.900
Rmeas	0.252	0.252
Number of reflections	233281	36696
<I/σ(I)>	8.49	0.84
Completeness [%]	99.3	97.6
Redundancy	10.1	10.1
CC(1/2)	0.997	0.467

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.18 M tri-ammonium citrate, 18% v/v PEG 3350, 0.01 M Cobalt (II) chloride hexahydrate