6UAY
Crystal structure of a GH128 (subgroup III) curdlan-specific exo-beta-1,3-glucanase from Blastomyces gilchristii (BgGH128_III)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | LNLS BEAMLINE W01B-MX2 |
Synchrotron site | LNLS |
Beamline | W01B-MX2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-08-09 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 1.458810 |
Spacegroup name | P 1 |
Unit cell lengths | 36.470, 48.720, 57.110 |
Unit cell angles | 102.61, 89.78, 93.89 |
Refinement procedure
Resolution | 47.430 - 1.800 |
R-factor | 0.1733 |
Rwork | 0.171 |
R-free | 0.21070 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6uaq |
RMSD bond length | 0.015 |
RMSD bond angle | 1.893 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.430 | 1.850 |
High resolution limit [Å] | 1.800 | 1.800 |
Number of reflections | 34823 | 2487 |
<I/σ(I)> | 13.25 | 4.29 |
Completeness [%] | 98.1 | |
Redundancy | 4.48 | |
CC(1/2) | 0.986 | 0.904 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.05 M Potassium di-hydrogen phosphate 20% Polyethylene glycol 8,000 |