6UAW
Crystal structure of a GH128 (subgroup II) endo-beta-1,3-glucanase from Pseudomonas viridiflava (PvGH128_II) in complex with laminaritriose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LNLS BEAMLINE W01B-MX2 |
| Synchrotron site | LNLS |
| Beamline | W01B-MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-07-10 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.239860 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 87.432, 33.871, 84.563 |
| Unit cell angles | 90.00, 113.11, 90.00 |
Refinement procedure
| Resolution | 27.180 - 1.500 |
| R-factor | 0.1581 |
| Rwork | 0.156 |
| R-free | 0.19220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6uav |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.953 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.180 | 1.590 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Number of reflections | 36122 | 5890 |
| <I/σ(I)> | 15.36 | 2.53 |
| Completeness [%] | 97.6 | |
| Redundancy | 6.39 | |
| CC(1/2) | 0.999 | 0.838 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 291 | bis-tris 0.1 M ammonium sulfate 2 M |
