6UAR

Crystal structure of a GH128 (subgroup I) endo-beta-1,3-glucanase from Amycolatopsis mediterranei (AmGH128_I) in complex with laminaritriose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	LNLS BEAMLINE W01B-MX2
Synchrotron site	LNLS
Beamline	W01B-MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-11-11
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.239850
Spacegroup name	P 1 21 1
Unit cell lengths	37.758, 77.980, 46.228
Unit cell angles	90.00, 102.45, 90.00

Refinement procedure

Resolution	36.900 - 1.400
R-factor	0.1698
Rwork	0.169
R-free	0.18170
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.014
RMSD bond angle	1.803
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.150	1.490
High resolution limit [Å]	1.400	1.400
Number of reflections	48504	7301
<I/σ(I)>	15.73
Completeness [%]	94.7
Redundancy	4.62
CC(1/2)	0.999	0.672

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	291	8% PEG20000 8% PEGMME 550 0.1 M sodium acetate 0.2 M magnesium chloride