6UAQ

Crystal structure of a GH128 (subgroup I) endo-beta-1,3-glucanase from Amycolatopsis mediterranei (AmGH128_I)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	LNLS BEAMLINE W01B-MX2
Synchrotron site	LNLS
Beamline	W01B-MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-11-23
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.033140
Spacegroup name	P 1 21 1
Unit cell lengths	37.350, 78.643, 46.183
Unit cell angles	90.00, 101.62, 90.00

Refinement procedure

Resolution	45.280 - 1.150
R-factor	0.1506
Rwork	0.150
R-free	0.16180
Structure solution method	MIRAS
RMSD bond length	0.018
RMSD bond angle	2.097
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELXDE
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.280	1.220
High resolution limit [Å]	1.150	1.150
Number of reflections	171671	24853
<I/σ(I)>	11.51
Completeness [%]	94.0
Redundancy	2.35
CC(1/2)	0.998	0.808

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	291	sodium acetate 0.1 M sodium chloride 1.5 M