6UAM
Apo-form Dimer of Y77A Mutant Putative Ryanodine Receptor from Bacteroides thetaiotaomicron VPI-5482
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-02-23 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97927 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 74.623, 89.993, 74.482 |
Unit cell angles | 90.00, 110.89, 90.00 |
Refinement procedure
Resolution | 37.806 - 2.802 |
R-factor | 0.1923 |
Rwork | 0.189 |
R-free | 0.25310 |
Structure solution method | SAD |
Data reduction software | HKL-3000 |
Data scaling software | SCALEPACK |
Phasing software | HKL-3000 |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.850 |
High resolution limit [Å] | 2.800 | 7.590 | 2.800 |
Rmerge | 0.105 | 0.052 | 0.665 |
Total number of observations | 72329 | ||
Number of reflections | 22375 | 1097 | 1135 |
<I/σ(I)> | 9.4 | ||
Completeness [%] | 98.4 | 93.2 | 99.7 |
Redundancy | 3.2 | 2.8 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 273 | 1.5M sodium citrate, 0.1M Bis-tris |