6U9Y
Wild-type MthK pore in 11 mM K+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-06-07 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.92 |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 63.811, 63.811, 44.050 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 31.900 - 1.800 |
| R-factor | 0.1963 |
| Rwork | 0.194 |
| R-free | 0.21660 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ldc |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.801 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.900 | 1.868 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.145 | 3.326 |
| Rmeas | 0.148 | 3.395 |
| Rpim | 0.029 | 0.673 |
| Number of reflections | 8824 | 838 |
| <I/σ(I)> | 22.59 | 1.26 |
| Completeness [%] | 99.8 | 98.7 |
| Redundancy | 24.9 | 25 |
| CC(1/2) | 1.000 | 0.613 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 1:1 protein (in 100 mM sodium chloride, 1 mM potassium chloride, 10 mM MOPS, pH 8.0) + 3.5-4.0 M 1,6-hexanediol, 100 mM MES-NaOH, 10 mM potassium chloride |
