6U9T

Wild-type MthK pore in 50 mM K+

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-06-07
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979
Spacegroup name	P 4 21 2
Unit cell lengths	63.811, 63.811, 44.050
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.900 - 1.550
R-factor	0.1941
Rwork	0.193
R-free	0.21020
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ldc
RMSD bond length	0.004
RMSD bond angle	0.588
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	31.910	1.607
High resolution limit [Å]	1.550	1.550
Rmerge	0.063	1.502
Rmeas	0.065	1.560
Rpim	0.013	0.416
Number of reflections	13679	1297
<I/σ(I)>	32.28
Completeness [%]	99.7	97.59
Redundancy	23.3	13.6
CC(1/2)	1.000	0.682

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	293	1:1 protein (in 100 mM sodium chloride, 1 mM potassium chloride, 10 mM MOPS, pH 8.0) + 3.5-4.0 M 1,6-hexanediol, 100 mM MES-NaOH, pH 6.75