6U9P

Wild-type MthK pore in ~150 mM K+

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-03-24
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.1
Spacegroup name	P 4 21 2
Unit cell lengths	63.811, 63.811, 44.050
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.050 - 1.650
R-factor	0.1852
Rwork	0.183
R-free	0.20750
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ldc
RMSD bond length	0.019
RMSD bond angle	1.272
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.050	1.710
High resolution limit [Å]	1.650	1.650
Rmerge	0.104	1.670
Rmeas	0.106	1.735
Rpim	0.022	0.467
Number of reflections	11414	1096
<I/σ(I)>	19.15
Completeness [%]	99.9	98.92
Redundancy	23.2	13.5
CC(1/2)	0.998	0.653

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	293	1:1 protein (in 100 mM potassium chloride, 10 mM MOPS-KOH, pH 8.0) + 3.5-4.0 M 1,6-hexanediol, 100 mM MES-KOH, pH 6.75