6U94

Structure of RND efflux system, outer membrane lipoprotein, NodT family from Burkholderia mallei ATCC 23344

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2019-04-18
Detector	RAYONIX MX-300
Wavelength(s)	0.97856
Spacegroup name	H 3 2
Unit cell lengths	93.440, 93.440, 370.790
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.480 - 2.350
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3d5k
RMSD bond length	0.002
RMSD bond angle	0.425
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MR-Rosetta
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.480	2.400
High resolution limit [Å]	2.350	2.350
Rmerge	0.051	0.615
Number of reflections	26543	1932
<I/σ(I)>	22.5	2.08
Completeness [%]	99.8
Redundancy	7.36
CC(1/2)	0.990	0.972

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	30 mM magnesium chloride hexahydrate, 30 mM calcium chloride dihydrate, 0.1 M Tris base, BICINE, pH 8.5, 20% v/v ethylene glycol, 10% w/v PEG8000