6U8U

RNA duplex bound with TNA 3'-3' imidazolium dimer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	99
Detector technology	CCD
Collection date	2018-06-15
Detector	MAR CCD 130 mm
Wavelength(s)	1
Spacegroup name	P 3 2 1
Unit cell lengths	43.956, 43.956, 84.516
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 2.400
R-factor	0.218
Rwork	0.216
R-free	0.26700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6c8d
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.490
High resolution limit [Å]	2.400	2.400
Rmerge	0.059	0.709
Rmeas	0.060	0.745
Rpim	0.020	0.228
Number of reflections	4009	387
<I/σ(I)>	37.2	3.1
Completeness [%]	99.9	100
Redundancy	10.2	10.6
CC(1/2)	1.000	0.958

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	291	10 % w/v Polyethylene glycol 6,000, 50 mM HEPES; pH 7.0, 200 mM Ammonium acetate, 150 mM Magnesium acetate