6U8T
Crystal structure of YopT domain of Pasteurella Multocida PfhB2-toxin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 80.15 |
| Detector technology | CCD |
| Collection date | 2017-10-22 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.9762 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.652, 62.957, 164.342 |
| Unit cell angles | 90.00, 93.15, 90.00 |
Refinement procedure
| Resolution | 29.410 - 2.390 |
| R-factor | 0.20156 |
| Rwork | 0.199 |
| R-free | 0.24467 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.508 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CRANK2 |
| Refinement software | REFMAC (5.8.0253) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.480 |
| High resolution limit [Å] | 2.390 | 2.390 |
| Rmeas | 0.088 | 0.587 |
| Number of reflections | 37166 | 3306 |
| <I/σ(I)> | 16.9 | 1.8 |
| Completeness [%] | 96.5 | 86.2 |
| Redundancy | 6.1 | 4.5 |
| CC(1/2) | 0.980 | 0.790 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2 M Magnesium Acetate and PEG 3350 22% |
