6U8D
Crystal structure of hepatitis C virus IRES junction IIIabc in complex with Fab HCV2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2018-03-11 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.979180 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 49.705, 161.223, 96.978 |
Unit cell angles | 90.00, 102.66, 90.00 |
Refinement procedure
Resolution | 61.362 - 1.807 |
R-factor | 0.1746 |
Rwork | 0.173 |
R-free | 0.20880 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | Fab HAVx 6mwn |
Data reduction software | HKL-2000 |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.14_3260)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 61.362 | 1.872 |
High resolution limit [Å] | 1.807 | 1.807 |
Rmerge | 0.040 | 1.134 |
Rmeas | 0.047 | |
Rpim | 0.023 | 0.669 |
Number of reflections | 67168 | 6420 |
<I/σ(I)> | 15.66 | 0.92 |
Completeness [%] | 98.7 | 94.58 |
Redundancy | 3.9 | 3.7 |
CC(1/2) | 0.999 | 0.481 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 295 | 0.2 M ammonium acetate, 0.1 M bis-tris pH 5.5, 45% 2-methyl-2,4-pentanediaol |