6U89
RNA duplex, bound with TNA monomer
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 99 |
Detector technology | CCD |
Collection date | 2017-06-09 |
Detector | MAR CCD 130 mm |
Wavelength(s) | 1 |
Spacegroup name | P 63 |
Unit cell lengths | 51.892, 51.892, 37.891 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 44.960 - 2.360 |
R-factor | 0.191 |
Rwork | 0.188 |
R-free | 0.27400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5hbw |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.440 |
High resolution limit [Å] | 2.360 | 2.360 |
Rmerge | 0.163 | 0.537 |
Rmeas | 0.185 | 0.621 |
Rpim | 0.086 | 0.307 |
Number of reflections | 2444 | 228 |
<I/σ(I)> | 10.67 | 1.89 |
Completeness [%] | 99.9 | 99.6 |
Redundancy | 4.5 | 3.9 |
CC(1/2) | 0.933 | 0.753 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 291 | 25 % v/v Polyethylene glycol 400, 100 mM tri-Sodium citrate; pH 5.6, 130 mM Sodium chloride, 60 mM Magnesium chloride |