6U83
OmpA-like domain of FopA1 from Francisella tularensis subsp. tularensis SCHU S4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-08-19 |
| Detector | DECTRIS PILATUS3 X 6M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 113.788, 134.971, 31.206 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.506 - 2.357 |
| R-factor | 0.2091 |
| Rwork | 0.207 |
| R-free | 0.23690 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5u1h |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.892 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX (dev_2947) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.390 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.145 | 1.157 |
| Number of reflections | 10225 | 528 |
| <I/σ(I)> | 26.25 | 1.96 |
| Completeness [%] | 98.9 | 99.6 |
| Redundancy | 11.9 | 10.6 |
| CC(1/2) | 0.999 | 0.899 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | SITTING DROP | 7.5 | 295 | 25% PEG3350, 0.2 M sodium chloride, 0.1 M HEPES, pH 7.5, cryoprotectant: paratone, SITTING DROP, TEMPERATURE 295K |
