6U7J
Uncultured Clostridium sp. Beta-glucuronidase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-12-07 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.0332 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 74.941, 105.655, 189.566 |
Unit cell angles | 90.00, 94.60, 90.00 |
Refinement procedure
Resolution | 29.310 - 2.200 |
R-factor | 0.1654 |
Rwork | 0.165 |
R-free | 0.20660 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4jkm |
RMSD bond length | 0.008 |
RMSD bond angle | 0.911 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.310 | 2.279 |
High resolution limit [Å] | 2.200 | 2.200 |
Number of reflections | 146335 | 14633 |
<I/σ(I)> | 10.89 | |
Completeness [%] | 97.9 | |
Redundancy | 2.7 | |
CC(1/2) | 0.997 | 0.834 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M Calcium Chloride, 0.1 M Tris: HCl, pH 8.5 and 20 % (w/v) PEG 4000 |