6U77
yGsy2p in complex with small molecule
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 193 |
| Detector technology | PIXEL |
| Collection date | 2017-03-26 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.9800 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 192.257, 206.557, 205.035 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 145.520 - 2.850 |
| R-factor | 0.2018 |
| Rwork | 0.199 |
| R-free | 0.25930 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3nb0 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.424 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 145.520 | 2.900 |
| High resolution limit [Å] | 2.850 | 2.850 |
| Rmeas | 0.100 | 0.843 |
| Rpim | 0.040 | 0.347 |
| Number of reflections | 95326 | 4703 |
| <I/σ(I)> | 20.3 | |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 6 | 5.7 |
| CC(1/2) | 0.857 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M Bis-Tris, pH 5.9 and 13-15% PEG300 |
