6U73
Human Angiopoietin-Like 4 C-Terminal Domain (cANGPTL4) with Myristic Acid
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-06-29 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.978 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 132.496, 135.632, 39.596 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.790 - 2.380 |
R-factor | 0.1989 |
Rwork | 0.194 |
R-free | 0.23980 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6eub |
RMSD bond length | 0.009 |
RMSD bond angle | 1.104 |
Data reduction software | HKL-3000 (1.16_3549) |
Data scaling software | HKL-3000 (1.16_3549) |
Phasing software | AutoSol |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.790 | 2.470 |
High resolution limit [Å] | 2.340 | 2.340 |
Rmerge | 0.090 | 0.408 |
Rpim | 0.039 | |
Number of reflections | 14821 | 712 |
<I/σ(I)> | 17.2 | |
Completeness [%] | 96.4 | 99.7 |
Redundancy | 6.3 | 6.5 |
CC(1/2) | 0.985 | 0.937 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 295 | 0.1M HEPES 7.5, 0.1M Sodium chloride, 1.6M Ammonium sulfate, Myristic acid |