6U6N
Structure of the trimeric globular domain of Adiponectin mutant - D187A Q188A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-04-17 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.033 |
Spacegroup name | H 3 |
Unit cell lengths | 75.704, 75.704, 48.865 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 39.180 - 2.150 |
R-factor | 0.2142 |
Rwork | 0.210 |
R-free | 0.25560 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6u66 |
RMSD bond length | 0.002 |
RMSD bond angle | 0.433 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.280 |
High resolution limit [Å] | 2.150 | 2.150 |
Rmeas | 0.730 | 0.660 |
Number of reflections | 29889 | 865 |
<I/σ(I)> | 12.42 | 1.59 |
Completeness [%] | 99.1 | 94.4 |
Redundancy | 5.3 | 3.04 |
CC(1/2) | 0.990 | 0.710 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 298 | 0.2 M sodium nitrate, 0.1 M MES pH 5.5, 20% PEG 3350 |