6U54

Anti-Zaire ebolavirus Nucleoprotein Single Domain Antibody Zaire C (ZC) Complexed with Zaire ebolavirus Nucleoprotein C-terminal Domain 634-739

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-10-21
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 43 21 2
Unit cell lengths	87.355, 87.355, 76.868
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	57.710 - 1.600
R-factor	0.1747
Rwork	0.172
R-free	0.21510
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6u51
RMSD bond length	0.009
RMSD bond angle	0.859
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	57.710	1.690
High resolution limit [Å]	1.600	1.600
Rmeas	0.054	1.886
Rpim	0.025	0.902
Number of reflections	38012	5651
<I/σ(I)>	13.5	0.8
Completeness [%]	96.2	98.9
Redundancy	4.2	4.2
CC(1/2)	0.999	0.499

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	1.1 M malonic acid, 0.15 M ammonium citrate tribasic, 0.072 M succinic acid, 0.18 M DL-malic acid, 0.24 M sodium acetate, 0.3 M sodium formate, 0.096 M ammonium tartrate dibasic