6U52
Anti-Sudan ebolavirus Nucleoprotein Single Domain Antibody Sudan B (SB) Complexed with Sudan ebolavirus Nucleoprotein C-terminal Domain 634-738
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2016-10-06 |
Detector | RIGAKU RAXIS HTC |
Wavelength(s) | 1.54178 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 173.977, 44.984, 66.438 |
Unit cell angles | 90.00, 105.94, 90.00 |
Refinement procedure
Resolution | 23.310 - 1.900 |
R-factor | 0.2004 |
Rwork | 0.198 |
R-free | 0.24340 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6u51 |
RMSD bond length | 0.008 |
RMSD bond angle | 0.948 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.140 | 2.000 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmeas | 0.082 | 1.573 |
Rpim | 0.044 | 0.849 |
Number of reflections | 38124 | 5705 |
<I/σ(I)> | 9.5 | 0.9 |
Completeness [%] | 96.9 | 99.8 |
Redundancy | 3.3 | 3.3 |
CC(1/2) | 0.998 | 0.471 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 295 | 10% polyethylene glycol 6000, 1.0 M lithium chloride, 0.1 M 2-(N-morpholino)ethanesulfonic acid (MES) pH 6.0 |