6U50
Anti-Sudan ebolavirus Nucleoprotein Single Domain Antibody Sudan B (SB)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2017-01-31 |
Detector | RIGAKU RAXIS HTC |
Wavelength(s) | 1.54178 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 47.839, 47.839, 85.378 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 37.270 - 1.600 |
R-factor | 0.1736 |
Rwork | 0.169 |
R-free | 0.21400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6u51 |
RMSD bond length | 0.009 |
RMSD bond angle | 0.961 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.690 | 1.680 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmeas | 0.142 | 1.820 |
Rpim | 0.045 | 0.605 |
Number of reflections | 15558 | 2140 |
<I/σ(I)> | 12.1 | 1.3 |
Completeness [%] | 99.4 | 96.2 |
Redundancy | 9.7 | 8.5 |
CC(1/2) | 0.999 | 0.367 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | 37.5% precipitant mix (2-methyl-2,4-pentanediol, polyethylene glycol [PEG] 1000, PEG 3350), 0.1 M amino acids mix (glutamate, alanine, glycine, lysine, serine), 0.1 M HEPES/MOPS pH 7.5 {Molecular Dimensions Morpheus HT-96 Condition H8} |