6U4B
WbbM bifunctional glycosytransferase apo structure
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-01-19 |
Detector | RAYONIX MX300HE |
Wavelength(s) | 0.97949 |
Spacegroup name | P 63 |
Unit cell lengths | 124.030, 124.030, 73.050 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 47.280 - 2.100 |
R-factor | 0.1902 |
Rwork | 0.188 |
R-free | 0.22690 |
Structure solution method | SAD |
RMSD bond length | 0.002 |
RMSD bond angle | 0.523 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.288 | 2.150 |
High resolution limit [Å] | 2.100 | 2.100 |
Number of reflections | 37124 | 2755 |
<I/σ(I)> | 14.7 | 2.05 |
Completeness [%] | 99.0 | |
Redundancy | 5.7 | |
CC(1/2) | 0.830 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 50 mM MgCl, 0.1 M HEPES and 30% v/v PEG MME 550 |