6U47

Coiled-coil Trimer with Glu:Ala:Lys Triad

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	ENRAF-NONIUS FR591
Temperature [K]	100
Detector technology	CCD
Collection date	2017-10-07
Detector	APEX II CCD
Wavelength(s)	1.5406
Spacegroup name	H 3
Unit cell lengths	39.015, 39.015, 98.163
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.856 - 2.301
R-factor	0.19
Rwork	0.183
R-free	0.24410
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	PROTEUM PLUS
Data scaling software	PROTEUM PLUS
Phasing software	PHENIX
Refinement software	PHENIX (dev_2906)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.856	2.383
High resolution limit [Å]	2.301	2.301
Rmerge	0.199	0.364
Rmeas	0.215	0.416
Rpim	0.080	0.200
Number of reflections	4941	240
<I/σ(I)>	8.5	1.6
Completeness [%]	99.6
Redundancy	5.8
CC(1/2)	0.892	0.849

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.2	298	50% PEG200, 100 mM sodium phosphate dibasic/potassium phosphate monobasic, pH 6.2