6U3V
Crystal structure of human alpha/epsilon-COP of the COPI vesicular coat bound to alpha-COP STM1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS-II BEAMLINE 17-ID-2 |
Synchrotron site | NSLS-II |
Beamline | 17-ID-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-07-24 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.97933 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 138.103, 138.103, 192.940 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.547 - 2.960 |
R-factor | 0.1748 |
Rwork | 0.172 |
R-free | 0.23080 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6tzt |
Data reduction software | XDS |
Data scaling software | Aimless (0.5.21) |
Phasing software | PHASER (2.8.2) |
Refinement software | PHENIX (1.13_2998: ???) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.550 | 3.030 |
High resolution limit [Å] | 2.956 | 2.960 |
Rmerge | 0.114 | 0.954 |
Rmeas | 0.120 | 1.005 |
Rpim | 0.038 | 0.313 |
Total number of observations | 31338 | |
Number of reflections | 45037 | 3157 |
<I/σ(I)> | 16.6 | 2.3 |
Completeness [%] | 99.6 | 96.3 |
Redundancy | 10.1 | 9.9 |
CC(1/2) | 0.999 | 0.784 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 300 | 0.1 M sodium citrate, pH 6.0, 0.05% (v/v) Tacsimate, pH 5.0, 11% (w/v) PEG 3350, 5 mM dithiothreitol Cryoprotected with 30% (v/v) ethylene glycol |