6U1Y
bcs1 AAA domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-01-01 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.206, 207.253, 116.615 |
| Unit cell angles | 90.00, 95.98, 90.00 |
Refinement procedure
| Resolution | 44.460 - 2.170 |
| R-factor | 0.1838 |
| Rwork | 0.181 |
| R-free | 0.23530 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | mBcs1-ATPgS |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.462 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.460 | 2.251 |
| High resolution limit [Å] | 2.170 | 2.170 |
| Rmerge | 0.077 | 0.699 |
| Number of reflections | 88747 | 7918 |
| <I/σ(I)> | 4.45 | |
| Completeness [%] | 86.8 | |
| Redundancy | 1.5 | |
| CC(1/2) | 0.977 | 0.499 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289 | 0.1M Hepes pH 7.5, 12% PEG 3350, 3% 1,6-hexanediol |
