6U1U

Human Angiopoietin-Like 4 C-Terminal Domain (cANGPTL4) with Palmitic Acid

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-29
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.988
Spacegroup name	C 2 2 21
Unit cell lengths	132.568, 135.300, 39.509
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.700 - 1.750
R-factor	0.1953
Rwork	0.194
R-free	0.21100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6eub
RMSD bond length	0.013
RMSD bond angle	1.289
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.700	1.780
High resolution limit [Å]	1.700	1.750
Rmerge	0.054	0.620
Rmeas	0.059	0.675
Rpim	0.023	0.264
Number of reflections	34756	1727
<I/σ(I)>	15.7
Completeness [%]	93.1
Redundancy	6.5
CC(1/2)	0.998	0.849

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	0.1 M HEPES 7.5 0.1 M Sodium chloride 1.6 M Ammonium sulfate Palmitic Acid