6U1P

Crystal structure of VpsU (VC0916) from Vibrio cholerae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-09-10
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1
Spacegroup name	I 41
Unit cell lengths	80.323, 80.323, 105.053
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	63.809 - 2.201
R-factor	0.1948
Rwork	0.188
R-free	0.25710
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4lrq
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	63.810	2.270
High resolution limit [Å]	2.200	2.200
Rmerge	0.068	0.724
Number of reflections	16904	1449
<I/σ(I)>	18.2	2.8
Completeness [%]	99.9
Redundancy	8.5
CC(1/2)	0.990	0.560

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	0.1 M HEPES (pH 7.5) 1.25 M tri-sodium citrate 10% glycerol