6U13
Crystal Structure of the metallo-beta-lactamase L1 from Stenotrophomonas maltophilia in the complex with the hydrolyzed antibiotic moxalactam
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2019-02-26 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.97919 |
| Spacegroup name | P 64 2 2 |
| Unit cell lengths | 103.918, 103.918, 98.929 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 35.830 - 1.520 |
| R-factor | 0.1292 |
| Rwork | 0.128 |
| R-free | 0.16040 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDBID 6U0Y |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.817 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.550 |
| High resolution limit [Å] | 1.520 | 1.520 |
| Rmerge | 0.095 | 0.980 |
| Number of reflections | 48271 | 1818 |
| <I/σ(I)> | 33.4 | 1.63 |
| Completeness [%] | 98.4 | 75.2 |
| Redundancy | 17.3 | |
| CC(1/2) | 0.999 | 0.631 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289 | 0.2 M citrate, 20 % (w/v) PEG 3350 |
