6U12
VHH R303 C33A/C102A in complex withthe LRR domain of InlB
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-02-14 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.9794 |
Spacegroup name | P 43 |
Unit cell lengths | 83.210, 83.210, 64.930 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 51.190 - 1.560 |
R-factor | 0.1758 |
Rwork | 0.174 |
R-free | 0.19890 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6dbf |
Data reduction software | xia2 |
Data scaling software | Aimless (0.7.1) |
Phasing software | PHASER |
Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 64.920 | 64.920 | 1.600 |
High resolution limit [Å] | 1.560 | 6.980 | 1.560 |
Rmerge | 0.063 | 0.028 | 0.613 |
Rmeas | 0.068 | 0.031 | 0.703 |
Rpim | 0.027 | 0.012 | 0.335 |
Total number of observations | 384474 | 4757 | 16105 |
Number of reflections | 62702 | 753 | 4302 |
<I/σ(I)> | 13.5 | 46.3 | 1.6 |
Completeness [%] | 99.3 | 99.9 | 92.8 |
Redundancy | 6.1 | 6.3 | 3.7 |
CC(1/2) | 0.999 | 0.999 | 0.592 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 300 | 0.49 M Sodium Phosphate monobasic monohydrate, 0.91 M Potassium Phosphate dibasic pH 6.8. |