6U0Z
Crystal Structure of the metallo-beta-lactamase L1 from Stenotrophomonas maltophilia in the complex with the hydrolyzed penicillin G
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2019-03-20 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.97916 |
| Spacegroup name | P 64 2 2 |
| Unit cell lengths | 104.759, 104.759, 98.434 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.300 - 1.650 |
| R-factor | 0.1587 |
| Rwork | 0.158 |
| R-free | 0.18060 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDBID 6U0Y |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.004 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.680 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.089 | 0.943 |
| Number of reflections | 38907 | 1892 |
| <I/σ(I)> | 44.7 | 4 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 20.8 | 16 |
| CC(1/2) | 0.894 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 289 | 0.1 M Bis-Tris pH 6.5, 2.0 M ammonium sulfate |
