6U0Y
Crystal Structure of the metallo-beta-lactamase L1 from Stenotrophomonas maltophilia
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-10-28 |
Detector | DECTRIS PILATUS3 X 6M |
Wavelength(s) | 0.97918 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 68.033, 97.229, 91.725 |
Unit cell angles | 90.00, 105.55, 90.00 |
Refinement procedure
Resolution | 48.630 - 1.700 |
R-factor | 0.1693 |
Rwork | 0.168 |
R-free | 0.19780 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDBID 5DPX |
RMSD bond length | 0.007 |
RMSD bond angle | 0.851 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.730 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.122 | 0.843 |
Number of reflections | 122234 | 6033 |
<I/σ(I)> | 23.1 | 2.38 |
Completeness [%] | 96.6 | |
Redundancy | 5.9 | |
CC(1/2) | 0.990 | 0.735 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 289 | 0.1 M HEPES pH 7.5, 10 %(w/v) PEG4000, 5 %(v/v) isopropanol |